sccmec type iv 1 3e 04 narsa nrs683 ga 298 1 3e 04 narsa nrs662  (ATCC)

Journal: Frontiers in Pharmacology

Article Title: Bakuchiol kills Staphylococcus aureus persisters and potentiates colistin activity against Acinetobacter baumannii persisters

doi: 10.3389/fphar.2025.1592183

Figure Lengend Snippet: Identification of the persister-killing activity of bakuchiol. (A) Screening results from a natural product library. Actively growing MRSA MW2 cells and MRSA MW2 persister cells were treated with 64 μg/mL of each natural product for 4 h. Each treated sample was then inoculated into fresh CaMH and incubated for 18 h, and the optical density at 600 nm (OD 600 ) was measured. Z-scores for regrowth of treated-growing and treated-persister cells were calculated by obtaining the OD 600 value of each sample, subtracting the mean OD 600 of all tested samples, and dividing by the standard deviation (SD) of the OD 600 values from all tested samples. (B) Chemical structure of bakuchiol. (C,D) Viability of MRSA MW2 (C) and VRSA VRS1 (D) persister cells treated with 100× MIC of conventional antibiotics—vancomycin (Van), gentamicin (Gm), ciprofloxacin (Cipro), daptomycin (Dap), and linezolid (Lin)—or the indicated concentrations of bakuchiol (BAK) for 4 h. Data points at the x-axis detection limit represent a CFU count of 2 × 10 2 CFU/mL. Individual data points (n = 3 biologically independent samples) are shown, with error bars representing the mean ± SD.

Article Snippet: The S. aureus strains MW2 BAA-1707 , ATCC 33591, and ATCC 43300; clinical MRSA isolates (HLSA 16278, 17064, 17078, 18380, 18807, 18840, 18883, 18888, 20835, and 21008) ( ); and vancomycin-resistant S. aureus (VRSA) strain VRS1 ( ) were grown in tryptic soy broth (TSB) (BD, Franklin Lakes, NJ, United States).

Techniques: Activity Assay, Incubation, Standard Deviation

Journal: Frontiers in Pharmacology

Article Title: Bakuchiol kills Staphylococcus aureus persisters and potentiates colistin activity against Acinetobacter baumannii persisters

doi: 10.3389/fphar.2025.1592183

Figure Lengend Snippet: Bakuchiol disrupts the MRSA persister membrane. (A) Uptake of SYTOX Green (Ex = 485 nm, Em = 525 nm) by MRSA MW2 persister cells treated with the indicated concentrations of bakuchiol (BAK). Results are presented as means (n = 3 biologically independent samples); error bars are omitted for clarity. RFU indicates relative fluorescence units. (B) Leakage of cellular ATP and proteins from MRSA MW2 persister cells treated with various BAK concentrations for 1 h. Leakage was measured using an ATP luminescence assay kit and a Micro BCA protein assay kit. Individual data points are shown; error bars represent means ± SD (n = 3). Statistical differences were analyzed using one-way analysis of variance with the post hoc Tukey test (**** p < 0.0001). (C) Changes in BAK MIC against MRSA MW2 in the presence of phosphatidylglycerol (PG), lysyl phosphatidylglycerol (Lysyl-PG), cardiolipin (CL), or lipopolysaccharides (LPS) were evaluated using checkerboard microdilution assays. The lipid component concentrations ranged from 0 to 64 μg/mL. The experiment was performed in triplicate, with all replicates showing consistent MIC changes.

Article Snippet: The S. aureus strains MW2 BAA-1707 , ATCC 33591, and ATCC 43300; clinical MRSA isolates (HLSA 16278, 17064, 17078, 18380, 18807, 18840, 18883, 18888, 20835, and 21008) ( ); and vancomycin-resistant S. aureus (VRSA) strain VRS1 ( ) were grown in tryptic soy broth (TSB) (BD, Franklin Lakes, NJ, United States).

Techniques: Membrane, Fluorescence, Luminescence Assay, Bicinchoninic Acid Protein Assay

Journal: Antibiotics

Article Title: Engineered Lysin-Derived Peptide as a Potent Antimicrobial for Acne Vulgaris

doi: 10.3390/antibiotics14040344

Figure Lengend Snippet: Bacterial strains used in this study.

Article Snippet: S. aureus , MW2 BAA-1707 , ATCC , USA400 pulsotype, MRSA; source: blood , [ ] .

Techniques:

Journal: Pharmaceuticals

Article Title: Chemical Composition, Biological Activity, and Potential Uses of Oregano ( Origanum vulgare L.) and Oregano Essential Oil

doi: 10.3390/ph18020267

Figure Lengend Snippet: Antimicrobial activity of O. vulgare L.

Article Snippet: , , Methicillin-resistant S. aureus : S. aureus ATCC 43300; S. aureus ATCC BAA-1707 , 0.25–0.5.

Techniques: Activity Assay, Inhibition, Extraction